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Anti-RAG2 Antibody EPRAGR239
Also for RAG2 (NM_009020)
|A recombinant protein fragment corresponding to residues in mouse RAG-2 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000
|Does not react with Rat, Human. Is unsuitable for IHC-P or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for IHC-P or IP.
|Mus musculus recombination activating gene 2 (Rag2)|
|RAG-2 is involved in the initiation of V(D)J recombination during B and T cell development. It forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in RAG-2 cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms (1).|
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Western blot - Anti-RAG2 antibody [EPRAGR239 ]; Anti-RAG2 antibody [EPRAGR239 ] at 1/1000 dilution + Mouse thymus lysate at 10 µg.Secondary.HRP labelled Goat anti Rabbit at 1/2000 dilution.Predicted band size : 59 kDa.
Western blot - Anti-RAG2 antibody [EPRAGR239 ]; All lanes : Anti-RAG2 antibody [EPRAGR239 ] at 1/1000 dilution.Lane 1 : Whole cell lysate of 293T transiently transfected with MBP-RAG2 core.Lane 2 : Whole cell lysate of 293T untransfected .Lane 3 : Whole cell lysate of thymus from wt Mouse (5 weeks old).Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti Rabbit at 1/2000 dilution.Predicted band size : 59 kDa.