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Anti-PSMA3 Antibody EPR5455
Also for PSMA3 (NM_152132)
|A synthetic peptide corresponding to residues in human PSMA3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 3 (PSMA3), transcript variant 2|
|The PSMA3 proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA3 is a member of the peptidase T1A family, a 20S core alpha subunit (1). |
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Western blot - Proteasome 20S alpha 3 antibody [EPR5455]; All lanes : Anti-Proteasome 20S alpha 3 antibody [EPR5455] at 1/1000 dilution.Lane 1 : A549 cell lysate.Lane 2 : JAR cell lysate.Lane 3 : PC12 cell lysate.Lane 4 : NIH3T3 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 28 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteasome 20S alpha 3 antibody [EPR5455]; ab109532, at 1/100 dilution, staining Proteasome 20S alpha 3 in paraffin embedded Human brain tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Proteasome 20S alpha 3 antibody [EPR5455]; ab109532, at 1/100 dilution, staining Proteasome 20S alpha 3 in HeLa cells by Immunofluorescence.