Anti-PRKAB1 Antibody, EP2181Y

Also for PRKAB1 (NM_006253)

OriGene Data

Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Serine 181 of human AMPK beta was used as immunogen.
Clone Name	EP2181Y	Isotype
Species Reactivity	Human	Concentration
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB: 1:2000 - 5000; ICC: 1:100 - 250; IP: 1:20;
Buffer	Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1)
Alternative Name	AMPK; HAMPKb
Database Link	NP_006244
Function	The 5-AMP-activated protein kinase (AMPK) is a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found, and the difference in expression patterns indicates tissue-specific roles for these isoforms (4). Phosphorylation on serine 24/25 and serine 182 have been linked to AMPK localization.
Related Pathway

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WB Image

Western blot analysis on human brain tissue lysate using anti-Phospho-AMPK beta 1 (pS181) RabMAb, 1:20,000 dilution. Cells were either (A) untreated (B) treated with Alkaline Phosphatase.

IHC Image

B. Immunohistochemical analysis of paraffin-embedded human skeletal muscle using anti-AMPK RabMAb(cat.#2271-1).

IF Image

C. Immunofluorescent staining of HeLa cells using anti-AMPK RabMAb.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-PRKAB1 Phospho Antibody EP2181Y

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data