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Anti-PRKAA1 PHOSPHO Antibody EPR5683
Also for PRKAA1 (NM_006251)
|A phospho specific peptide corresponding to residues surrounding Threonine 183 of human AMPK alpha 1 and Threonine 172 of human AMPK alpha 2 was used as an immunogen. This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for ICC/IF or IHC-P.
|Homo sapiens protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1|
|The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1) AMPK is a heterotrimeric protein comprising a (63 kDa), ß (38 kDa) and ? (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.|
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Western blot - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683]; All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) [EPR5683] antibody at 1/1000 dilution.Lane 1 : 293 cell lysate.Lane 2 : Lysate of 293 cells treated with lambda phosphatase.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 64 kDa.
Other-Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683](TA310776); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-AMPK alpha 1 (pT183) + AMPK alpha 2 (pT172) antibody [EPR5683]; Overlay histogram showing HeLa cells stained with TA310776 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.