Anti-NPC1 Antibody, EPR5209

Also for NPC1 (NM_000271)

OriGene Data

Immunogen	A synthetic peptide corresponding to residues at the C-terminus of human NPC1 was used as an immunogen.
Clone Name	EPR5209	Isotype
Species Reactivity	Human, Mouse, Rat	Concentration
Guaranteed Application *	WB, IHC	Suggested Dilutions	WB: 1:1,000 - 10,000; IHC 1:50 - 100;
Buffer	Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens Niemann-Pick disease, type C1 (NPC1)
Alternative Name	NPC
Database Link	NP_000262
Function	NPC1 is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this protein cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by overaccumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments (1).
Related Pathway

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WB Image

Western blot analysis on (A) 3T3-L1, (B) L6, (C) HepG2, (D) THP-1, (E) 293T, (F) PC-3, (G) rat liver, and (H) rat brain lysates using anti-NPC1 RabMAb.

IHC Image

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NPC1 RabMAb.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-NPC1 Antibody EPR5209

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data