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Anti-NPC1 Antibody EPR5209
Also for NPC1 (NM_000271)
|A synthetic peptide corresponding to residues at the C-terminus of human NPC1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens Niemann-Pick disease, type C1 (NPC1)|
|NPC1 is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this protein cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by overaccumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments (1).|
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Western blot - Anti-Niemann Pick C1 antibody [EPR5209]; All lanes : Anti-Niemann Pick C1 antibody [EPR5209] at 1/1000 dilution.Lane 1 : 3T3 L1 cell lysate.Lane 2 : L6 cell lysate.Lane 3 : HepG2 cell lysate .Lane 4 : THP1 cell lysate .Lane 5 : 293T cell lysate.Lane 6 : PC3 cell lysate .Lane 7 : Rat liver lysate .Lane 8 : Rat brain lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 142 kDa.Observed band size : 180 kDa .
Other-Anti-Niemann Pick C1 antibody [EPR5209](TA310727); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C1 antibody [EPR5209]; Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Niemann Pick C1 with TA310727 at 1/50.