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Anti-NEK6 Antibody EPR5283
Also for NEK6 (NM_001145001)
|A synthetic peptide corresponding to residues in human NEK6 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens NIMA-related kinase 6 (NEK6), transcript variant 1|
|NEK6 is a member of the mammalian NIMA-related kinases (1). It is involved in the control of mitotic spindle formation. NEK6 kinase activity is up-regulated in M phase. Expression of an inactive NEK6 mutant arrested cells in M phase and interfered with chromosome segregation. It is suggested that NEK6 is required for cell cycle progression through mitosis and demonstrate that loss of NEK6 function leads to defective mitosis and apoptosis (2).|
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Western blot - Anti-NEK6 antibody [EPR5283]; All lanes : Anti-NEK6 antibody [EPR5283] at 1/1000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : 293T cell lysate.Lane 4 : HT1080 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 36 kDa.