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Anti-NDRG1 PHOSPHO Antibody EPR5594
Also for NDRG1 (NM_006096)
|A phospho specific peptide corresponding to residues surrounding Serine 330 of human NDRG1 was used as an immunogen. This antibody only detects NDRG1 phosphorylated at Serine 330.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens N-myc downstream regulated 1 (NDRG1), transcript variant 2|
|CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5|
|NDRG1 is a member of the N-myc downregulated family that belongs to the alpha/beta hydrolase superfamily. It is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. NDRG1 is necessary for p53-mediated caspase activation and apoptosis. Mutations in NDRG1 have been reported to be causative for hereditary motor and sensory neuropathy-Lom (1).|
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Western blot - Anti-NDRG1 (phospho S330) antibody [EPR5594]; All lanes : Anti-NDRG1 (phospho S330) antibody [EPR5594] at 1/10000 dilution.Lane 1 : Untreated 293T cell lysates.Lane 2 : Calyculin A treated 293T cell lysates.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 43 kDa.
Other-Anti-NDRG1 (phospho S330) antibody [EPR5594](ab124713); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 (phospho S330) antibody [EPR5594]; ab124713, at 1/100, staining NDRG1 in paraffin embedded Human kidney tissue by Immunohistochemistry.