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Anti-METAP2 Antibody EPR6886(B)
Also for METAP2 (NM_006838)
|A synthetic peptide corresponding to residues in human METAP2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100; IHC-Fr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC/IF,IHC-P or IP.
|Homo sapiens methionyl aminopeptidase 2 (METAP2)|
|MAP2; MNPEP; p67; p67eIF2|
|METAP2 is a member of the methionyl aminopeptidase family and binds 2 cobalt or manganese ions. It functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of METAP2 is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site (1). |
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Western blot - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)]; All lanes : Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] at 1/1000 dilution.Lane 1 : HepG2 cell lysates.Lane 2 : 293T cell lysates.Lane 3 : HeLa cell lysates.Lane 4 : C6 cell lysates.Lane 5 : RAW 264.7 cell lysates.Lane 6 : PC12 cell lysates.Lane 7 : NIH 3T3 cell lysates.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 52 kDa.Observed band size : 67 kDa .
Immunohistochemistry (Frozen sections) - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)]; ab124953 staining Methionine Aminopeptidase 2 in Mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with Acetone and blocked with 5% Serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/25 in PBS + 5% goat serum) for 1 hours at 25°C. An Alexa Fluor?488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
Flow Cytometry - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)]; ab124953 at 1/10 dilution staining Methionine Aminopeptidase 2 in permeabilized 293T cells by Flow cytometry (red) or a Rabbit IgG (negative control) (green).