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Anti-MCM4 Antibody EPR5245
Also for MCM4 (NM_005914)
|A synthetic peptide corresponding to residues near the C-terminus of human MCM4 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, FC
||WB: 1:50000 - 1:200000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
|Does not react with Rat. Is unsuitable for ICC/IF or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC/IF or IHC-P.
|Homo sapiens minichromosome maintenance complex component 4 (MCM4), transcript variant 1|
|CDC21; CDC54; hCdc21; NKCD; NKGCD; P1-CDC21|
|MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of MCM4 by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex (1). |
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Western blot - Anti-MCM4 antibody [EPR5245]; All lanes : Anti-MCM4 antibody [EPR5245] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Molt-4 cell lysate.Lane 3 : A431 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 97 kDa.
Other-Anti-MCM4 antibody [EPR5245](ab124836); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-MCM4 antibody [EPR5245]; Flow cytometric analysis of permeabilized Molt-4 cells using anti-MCM4 in red or a rabbit IgG in green(negative).