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Anti-MAPK1 PHOSPHO Antibody EPR5463
Also for MAPK1 (NM_002745)
|A phospho specific peptide corresponding to residues surrounding Threonine 215 of human MARK1 was used as an immunogen. This antibody only detects MARK1 phosphorylated at Threonine 215.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
|Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1|
|ERK; ERK2; ERT1; MAPK2; p38; p40; p41; p41mapk; P42MAPK; PRKM1; PRKM2|
|MARK belongs to the AMPK (adenosine-monophosphate activated protein kinase) branch of the CAMK (calcium/calmodulin-dependent protein kinase) group of kinases (1). MARK1 has an important neuronal function, like other MARK proteins (2). It phosphorylates Tau and related microtubule-associated proteins (MAPs) at specific sites in the microtubule binding repeats. Phosphorylation of Tau by MARK1 results in the detachment from microtubules and affects microtubule dynamics and microtubule-based intracellular transport (3). MARK1 is activated by phosphorylation on Threonine 215 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39 (4). Moreover, hyperphosphorylation by MARK1 is implicated in the initial step for the abnormal aggregation of Tau in Alzheimer’s disease (3).|
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
Senescence and Autophagy
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway
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Western blot - Anti-MARK1 (phospho T215) antibody [EPR5463]; All lanes : Anti-MARK1 (phospho T215) antibody [EPR5463] at 1/10000 dilution.Lane 1 : SH-SY5Y cell lysate, untreated.Lane 2 : SH-SY5Y cell lysate, treated with Okadaic acid and Calyculin A .Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 89 kDa.
Other-Anti-MARK1 (phospho T215) antibody [EPR5463](ab126731); Equilibrium disassociation constant (KD)..