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Home Antibody All anti-LIN28A antibodies

Anti-LIN28A Antibody EPR4640

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA310658
  • Rabbit Monoclonal antibody against LIN28A
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC411135) , 20ug Explanation
100ul $325 In Stock
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WB(1)
IHC(5)
IF(1)
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Also for LIN28A (NM_024674)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human LIN28A was used as an immunogen.
Clone NameEPR4640 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 23 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens lin-28 homolog A (C. elegans) (LIN28A)
Alternative NameCSDD1; LIN-28; lin-28A; LIN28; ZCCHC1
Database LinkNP_078950
Entrez Gene 79727 Human
FunctionLIN28A, a promoter of pluripotency, is a developmentally regulated RNA binding protein, which selectively blocks the processing of pri-let-7 miRNAs in embryonic cells (1,2). One of the let-7 family members, let-7g targets and represses LIN28 through four 3'-UTR binding sites. It has been reported that LIN28/let-7 pair has the potential to act as a 'toggle switch' that balances the decision to maintain pluripotency vs. differentiation (3). LIN28 was identified as a negative regulator of miRNA biogenesis and suggested that it may play a central role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (1).
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-Lin28A antibody [EPR4640]; All lanes : Anti-Lin28A antibody [EPR4640] at 1/10000 dilution.Lane 1 : NCCIT cell lysate.Lane 2 : JAR cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 23 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640]; TA310658, at 1/100 dilution, staining Lin28A in paraffin-embedded Human seminoma tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Lin28A antibody [EPR4640](TA310658); TA310658 showing positive staining in Glioma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Lin28A antibody [EPR4640](TA310658); TA310658 showing positive staining in Dysgerminoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Lin28A antibody [EPR4640](TA310658); TA310658 showing positive staining in Embryonal carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Lin28A antibody [EPR4640](TA310658); TA310658 showing negative staining in Normal brain tissue.
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Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640]; TA310658, at 1/100 dilution, staining Lin28A in NCCIT cells by Immunofluorescence.
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