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Anti-IL1RN Antibody EPR6483
Also for IL1RN (NM_000577)
|A synthetic peptide corresponding to residues on the C-terminus in human IL-1RA was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, FC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Homo sapiens interleukin 1 receptor antagonist (IL1RN), transcript variant 3|
|DIRA; ICIL-1RA; IL-1ra; IL-1ra3; IL-1RN; IL1F3; IL1RA; IRAP; MVCD4|
|IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A mutation of IL-1RA is reported to be associated with increased risk of osteoporotic fractures and gastric cancer (1). |
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Western blot - Anti-IL1RA antagonist antibody [EPR6483]; All lanes : Anti-IL1RA antibody [EPR6483] at 1/10000 dilution.Lane 1 : A431 cell lysates.Lane 2 : 293T cell lysates.Lane 3 : MOLT4 cell lysates.Lane 4 : HeLa cell lysates.Lane 5 : RAW264.7 cell lysates.Lane 6 : NIH 3T3 cell lysates.Lane 7 : L6 cell lysates.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 20 kDa.
Other-Anti-IL1RA antibody [EPR6483](TA310622); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL1RA antibody [EPR6483]; TA310622, at a 1/100 dilution, staining IL1RA in paraffin embedded Human kidney tissue by Immunohistochemistry.
Flow Cytometry - Anti-IL1RA antibody [EPR6483]; Overlay histogram showing A431 cells stained with TA310622 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.