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Anti-IL11RA Antibody EPR5446
Also for IL11RA (NM_001142784)
|A synthetic peptide corresponding to residues in human IL-11R alpha was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; FC: 1:100 - 1:1000; ICC/IF: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for IP.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Is unsuitable for IP.
|Homo sapiens interleukin 11 receptor, alpha (IL11RA), transcript variant 3|
|Interleukin 11 is a stromal cell-derived cytokine that belongs to a family of pleiotropic and redundant cytokines that use the gp130 transducing subunit in their high affinity receptors. IL-11R alpha is a member of the hematopoietic cytokine receptor family. This particular receptor is very similar to ciliary neurotrophic factor, since both contain an extracellular region with a 2-domain structure composed of an immunoglobulin-like domain and a cytokine receptor-like domain (1). |
Cytokine-cytokine receptor interaction
Hematopoietic cell lineage
Jak-STAT signaling pathway
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Western blot - Anti-IL11RA antibody [EPR5446]; All lanes : Anti-IL11RA antibody [EPR5446] at 1/1000 dilution.Lane 1 : 293T lysate.Lane 2 : Saos2 lysate.Lane 3 : A673 lysate.Lane 4 : PC3 lysate.Lane 5 : Hut78 lysate.Lane 6 : K562 lysate.Lane 7 : TF1 lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 45 kDa.Observed band size : 45 kDa.
Other-Anti-IL11RA antibody [EPR5446](TA310615); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL11RA antibody [EPR5446]; TA310615 at 1/500 staining IL11RA in paraffin embedded Human Kidney tissue by immunohistochemistry.
Flow Cytometry - Anti-IL11RA antibody [EPR5446]; Overlay histogram showing K562 cells stained with TA310615 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.