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Home Antibody All anti-IL11RA antibodies

Anti-IL11RA Antibody EPR5446

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310615
  • Rabbit Monoclonal antibody against IL-11R alpha (IL11RA)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC428277) , 20ug
100ul $325 Next Day
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WB(1)
IHC(1)
FC(1)
Assay(1)
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Also for IL11RA (NM_001142784)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human IL-11R alpha was used as an immunogen.
Clone NameEPR5446 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; FC: 1:100 - 1:1000; ICC/IF: 1:250 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens interleukin 11 receptor, alpha (IL11RA), transcript variant 3
Alternative NameCRSDA
Database LinkNP_001136256
FunctionInterleukin 11 is a stromal cell-derived cytokine that belongs to a family of pleiotropic and redundant cytokines that use the gp130 transducing subunit in their high affinity receptors. IL-11R alpha is a member of the hematopoietic cytokine receptor family. This particular receptor is very similar to ciliary neurotrophic factor, since both contain an extracellular region with a 2-domain structure composed of an immunoglobulin-like domain and a cytokine receptor-like domain (1).
Related Pathway
Cytokine-cytokine receptor interaction
Hematopoietic cell lineage
Jak-STAT signaling pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-IL11RA antibody [EPR5446]; All lanes : Anti-IL11RA antibody [EPR5446] at 1/1000 dilution.Lane 1 : 293T lysate.Lane 2 : Saos2 lysate.Lane 3 : A673 lysate.Lane 4 : PC3 lysate.Lane 5 : Hut78 lysate.Lane 6 : K562 lysate.Lane 7 : TF1 lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 45 kDa.Observed band size : 45 kDa.
Assay Image
Other-Anti-IL11RA antibody [EPR5446](TA310615); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL11RA antibody [EPR5446]; TA310615 at 1/500 staining IL11RA in paraffin embedded Human Kidney tissue by immunohistochemistry.
FC Image
Flow Cytometry - Anti-IL11RA antibody [EPR5446]; Overlay histogram showing K562 cells stained with TA310615 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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