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Home Antibody All anti-HYOU1 antibodies

Anti-HYOU1 Antibody EP5891

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310602
  • Rabbit Monoclonal antibody against GRP170 / ORP150 (HYOU1)
100ul 325 3-7 Days
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WB(1)
IHC(2)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human GRP170 / ORP150 was used as an immunogen.
Clone NameEP5891 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsIP: 1:10 - 1:100; ICC/IF: 1:250 - 1:500; WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens hypoxia up-regulated 1 (HYOU1), transcript variant 2
Alternative NameGRP-170; Grp170; HSP12A; ORP-150; ORP150
Database LinkNP_001124463
FunctionGRP170 / ORP150 belongs to the heat shock protein 70 family. It uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. GRP170 / ORP150 is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. GRP170 / ORP150 also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and mitochondria by an amino-terminal targeting signal (1).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-ORP150 antibody [EP5891]; All lanes : Anti-ORP150 antibody [EP5891] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : HepG2 cell lysate.Lane 5 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.developed using the ECL technique.Predicted band size : 111 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891]; Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling ORP150 using TA310602 at a 1/250 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891]; Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling ORP150 using TA310602 at a 1/250 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-ORP150 antibody [EP5891]; Immunofluorescence staining of MCF7 cells, labelling ORP150 using TA310602 at a 1/250 dilution (red). Cell nuclei are shown in blue.

 

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