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Anti-HYOU1 Antibody EP5891
Also for HYOU1 (NM_001130991)
|A synthetic peptide corresponding to residues near the C-terminus of human GRP170 / ORP150 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||IP: 1:10 - 1:100; ICC/IF: 1:250 - 1:500; WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens hypoxia up-regulated 1 (HYOU1), transcript variant 2|
|GRP-170; Grp170; HSP12A; ORP-150; ORP150|
|GRP170 / ORP150 belongs to the heat shock protein 70 family. It uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. GRP170 / ORP150 is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. GRP170 / ORP150 also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and mitochondria by an amino-terminal targeting signal (1).|
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Western blot - Anti-ORP150 antibody [EP5891]; All lanes : Anti-ORP150 antibody [EP5891] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : HepG2 cell lysate.Lane 5 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.developed using the ECL technique.Predicted band size : 111 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891]; Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling ORP150 using ab134944 at a 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891]; Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling ORP150 using ab134944 at a 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ORP150 antibody [EP5891]; Immunofluorescence staining of MCF7 cells, labelling ORP150 using ab134944 at a 1/250 dilution (red). Cell nuclei are shown in blue.