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Anti-HMGB2 Antibody EPR6301
Also for HMGB2 (NM_001130688)
|A synthetic peptide corresponding to residues on the N-terminus in human HMGB2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens high mobility group box 2 (HMGB2), transcript variant 2|
|HMGB2 is a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. HMGB2 was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination (1).|
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Western blot - Anti-HMGB2 antibody [EPR6301]; All lanes : Anti-HMGB2 antibody [EPR6301] at 1/10000 dilution.Lane 1 : K562 cell lysates.Lane 2 : HeLa cell lysates.Lane 3 : PC12 cell lysates.Lysates/proteins at 10 µg per lane.Secondary.Goat-anti-rabbit HRP at 1/2000 dilution.Predicted band size : 24 kDa.
Other-Anti-HMGB2 antibody [EPR6301](ab124670); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB2 antibody [EPR6301]; ab124670, at a 1/250 dilution, staining HMGB2 in paraffin embedded Human breast tissue by Immunohistochemistry.