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Home Antibody All anti-HIST4H4 antibodies

Anti-HIST4H4 Antibody EPR1004

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310577
  • Rabbit Monoclonal antibody against Histone H4 Acetyl (K16) (HIST4H4)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC406378) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(2)
IF(1)
FC(1)
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Also for HIST4H4 (NM_175054)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lysine 16 of human Histone H4 was used as an immunogen. The antibody only detects Histone H4 acetylated on Lysine 16.
Clone NameEPR1004 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:250 - 1:500; FC: 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens histone cluster 4, H4 (HIST4H4)
Alternative NameH4/p
Database LinkNP_778224
Entrez Gene 121504 Human
Entrez Gene 320332 Mouse
FunctionChanges in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of histone H4 thus appears to play a primary role in the structural changes that mediate enhanced binding of transcription factors to their recognition sites within nucleosomes (3). Histone H4 can be reversibly acetylated at lysine residues 5, 8, 12 and 16. It has been shown that, in H4 from human cells, the four lysine residues are acetylated in a preferred, but not exclusive order, namely lysine 16, followed by 12 and 8, followed by 5 (4).
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H4 antibody [EPR1004]; All lanes : Anti-Histone H4 (acetyl K16) [EPR1004] antibody at 1/1000 dilution.Lane 1 : HeLa cell lysates, untreated .Lane 2 : HeLa cell lysates treated with TSA.Lysates/proteins at 10 ug per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 11 kDa.Observed band size : 11 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H4 antibody [EPR1004]; TA310577, at a1/100 dilution, staining Histone H4 in paraffin embedded Human testis tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H4 antibody [EPR1004]; TA310577, at a 1/100 dilution, staining Histone H4 in paraffin embedded Human transitional cell carcinoma tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H4 antibody [EPR1004]; TA310577, at a 1/250 dilution, staining Histone H4 in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry - Anti-Histone H4 (acetylated) (acetyl K16) antibody [EPR1004]; Overlay histogram showing HeLa cells stained with TA310577 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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