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Anti-HIST4H4 Antibody EPR1004
Also for HIST4H4 (NM_175054)
|A synthetic acetylated peptide corresponding to residues surrounding Lysine 16 of human Histone H4 was used as an immunogen. The antibody only detects Histone H4 acetylated on Lysine 16.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:250 - 1:500; FC: 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens histone cluster 4, H4 (HIST4H4)|
|Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of histone H4 thus appears to play a primary role in the structural changes that mediate enhanced binding of transcription factors to their recognition sites within nucleosomes (3). Histone H4 can be reversibly acetylated at lysine residues 5, 8, 12 and 16. It has been shown that, in H4 from human cells, the four lysine residues are acetylated in a preferred, but not exclusive order, namely lysine 16, followed by 12 and 8, followed by 5 (4).|
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Western blot - Histone H4 antibody [EPR1004]; All lanes : Anti-Histone H4 (acetyl K16) [EPR1004] antibody at 1/1000 dilution.Lane 1 : HeLa cell lysates, untreated .Lane 2 : HeLa cell lysates treated with TSA.Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 11 kDa.Observed band size : 11 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H4 antibody [EPR1004]; ab109463, at a1/100 dilution, staining Histone H4 in paraffin embedded Human testis tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H4 antibody [EPR1004]; ab109463, at a 1/100 dilution, staining Histone H4 in paraffin embedded Human transitional cell carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Histone H4 antibody [EPR1004]; ab109463, at a 1/250 dilution, staining Histone H4 in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-Histone H4 (acetylated) (acetyl K16) antibody [EPR1004]; Overlay histogram showing HeLa cells stained with ab109463 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.