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Home Antibody All anti-HIST4H4 antibodies

Anti-HIST4H4 Antibody EPR1004


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA310577 Rabbit Monoclonal antibody against Histone H4 Acetyl (K16) (HIST4H4) 100ul $325 In Stock
LC406378 HIST4H4 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for HIST4H4 (NM_175054)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lysine 16 of human Histone H4 was used as an immunogen. The antibody only detects Histone H4 acetylated on Lysine 16.
Clone NameEPR1004 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:250 - 1:500; FC: 1:1000
Predicted MW Explanation 11 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens histone cluster 4, H4 (HIST4H4)
Alternative NameH4/p
Database LinkNP_778224
Entrez Gene 121504 Human
Entrez Gene 320332 Mouse
FunctionChanges in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of histone H4 thus appears to play a primary role in the structural changes that mediate enhanced binding of transcription factors to their recognition sites within nucleosomes (3). Histone H4 can be reversibly acetylated at lysine residues 5, 8, 12 and 16. It has been shown that, in H4 from human cells, the four lysine residues are acetylated in a preferred, but not exclusive order, namely lysine 16, followed by 12 and 8, followed by 5 (4).
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Histone H4 antibody [EPR1004]; All lanes : Anti-Histone H4 (acetyl K16) [EPR1004] antibody at 1/1000 dilution.Lane 1 : HeLa cell lysates, untreated .Lane 2 : HeLa cell lysates treated with TSA.Lysates/proteins at 10 ug per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 11 kDa.Observed band size : 11 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H4 antibody [EPR1004]; TA310577, at a1/100 dilution, staining Histone H4 in paraffin embedded Human testis tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H4 antibody [EPR1004]; TA310577, at a 1/100 dilution, staining Histone H4 in paraffin embedded Human transitional cell carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Histone H4 antibody [EPR1004]; TA310577, at a 1/250 dilution, staining Histone H4 in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-Histone H4 (acetylated) (acetyl K16) antibody; Overlay histogram showing HeLa cells stained with TA310577 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control.


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