Home Antibody All anti-HIST1H3J antibodies
Anti-HIST1H3J PHOSPHO Antibody EPR5930
Also for HIST1H3J (NM_003535)
|A phospho specific peptide corresponding to residues surrounding Threonine 11 of human Histone H3 was used as an immunogen. This antibody only detects Histone H3 phosphorylated at Theronine 11.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000
|Does not react with Mouse. Is unsuitable for Flow Cyt,ICC,IHC-P or IP.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens histone cluster 1, H3j (HIST1H3J)|
|Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Threonine 11 occurs preferentially at the centromere from prophase to anaphase (5).|
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Western blot - Anti-Histone H3 (phospho T11) antibody [EPR5930]; All lanes : Anti-Histone H3 (phospho T11) antibody [EPR5930] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate treated with FBS and Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 15 kDa.Observed band size : 17 kDa .