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Anti-HINT1 Antibody EPR5108
Also for HINT1 (NM_005340)
|A synthetic peptide corresponding to residues in human Hint1 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; FC: 1:100 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Homo sapiens histidine triad nucleotide binding protein 1 (HINT1), transcript variant 1|
|HINT; NMAN; PKCI-1; PRKCNH1|
|Hint1, or histidine triad nucleotide-binding protein 1, belongs to the first branch of the HIT superfamily (1). It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. Hint1 also inhibits the activity of the transcription factors AP1, MITF and USF2, as well as influences the transcription process of the Wnt/beta-catenin pathway (2). In addition, mammalian Hint1 has been suggested to have some tumor suppressor activity (3).|
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Western blot - Anti-HINT1 antibody [EPR5108]; All lanes : Anti-HINT1 antibody [EPR5108] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 14 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108]; TA310574, at 1/250 dilution, staining HINT1 in paraffin embedded breast carcinoma tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108]; TA310574, at 1/250 dilution, staining HINT1 in paraffin embedded colonic adenocarcinoma tissue by immunohistochemistry.