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Anti-GTF2F2 Antibody EPR7584
Also for GTF2F2 (NM_004128)
|A synthetic peptide corresponding to residues in human TFIIF was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; ICC/IF: 1:25 - 1:50
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2)|
|BTF4; RAP30; TF2F2; TFIIF|
|TFIIF functions in both transcription initiation and elongation and is one of three general factors, TFIIB, TFIIE, and TFIIF, that directly binds RNA polymerase II (Pol II). TFIIF facilitates Pol II incorporation into a PIC intermediate-containing promoter DNA, TBP, and TFIIB and is required for subsequent recruitment of TFIIE and TFIIH. TFIIF also has functions in open complex formation, transcription start site selection, promoter escape, stabilization of an RNA–DNA hybrid within Pol II, and, along with TFIIS, stimulates elongation by purified Pol II (1).|
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Western blot - Anti-GTF2F2 antibody [EPR7584]; All lanes : Anti-GTF2F2 antibody [EPR7584] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : 30 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584]; ab128945 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584]; ab128945 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human colon tissue by immunohistochemistry.