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Anti-GK Antibody EPR6567
|A synthetic peptide corresponding to residues in human Glycerol Kinase was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC-P.
|Homo sapiens glycerol kinase (GK), transcript variant 1|
|Glycerol Kinase belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in Glycerol Kinase are associated with glycerol kinase deficiency (GKD) (1). |
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Western blot - Anti-Glycerol kinase antibody [EPR6567]; All lanes : Anti-Glycerol kinase antibody [EPR6567] at 1/1000 dilution.Lane 1 : Mouse adipose tissue lysate.Lane 2 : Mouse adipose tissue lysate.Lysates/proteins at 25 µg per lane.Secondary.HRP-conjugated Donkey anti-rabbit polyclonal at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 61 kDa.Observed band size : 55 kDa .Exposure time : 5 minutesThis image is courtesy of an anonymous Abreview
Western blot - Anti-Glycerol kinase antibody [EPR6567]; All lanes : Anti-Glycerol kinase antibody [EPR6567] at 1/1000 dilution.Lane 1 : Fetal liver lysate.Lane 2 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 61 kDa.Observed band size : 55 kDa .
Other-Anti-Glycerol kinase antibody [EPR6567](ab126599); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-Glycerol kinase antibody [EPR6567]; ab126599, at 1/100 dilution, staining Glycerol kinase in permeabilized HepG2 cells by Immunofluorescence (red). A rabbit IgG is used as a negative control (green).