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Anti-GFPT1 Antibody EPR4854
Also for GFPT1 (NM_002056)
|A synthetic peptide corresponding to residues in human GFAT1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or ICC.
|Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 2|
|CMSTA1; GFA; GFAT; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1L; MSLG|
|GFAT1 is the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. It catalyzes the formation of glucosamine 6-phosphate (1). The GFAT-mediated amino sugar pathway has been shown to be involved in the desensitization of adipocytes to insulin by glucose in non-insulin-dependent diabetes mellitus (2). It is most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins (3).|
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Western blot - Anti-GFPT1 antibody [EPR4854]; All lanes : Anti-GFPT1 antibody [EPR4854] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : JAR cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : Human placenta lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.developed using the ECL technique.Predicted band size : 79 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854]; ab125069, at a 1/100 dilution, staining GFPT1 in paraffin-embedded Human testis tissue by immunohistochemistry.