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Anti-GCLM Antibody EPR6667
Also for GCLM (NM_002061)
|A synthetic peptide corresponding to residues in human GCLM was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; FC: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC.
|Homo sapiens glutamate-cysteine ligase, modifier subunit (GCLM)|
|GCLM (Glutamate-cysteine ligase) is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLM deficiency has been implicated in some forms of hemolytic anemia (1).|
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Western blot - Anti-GCLM antibody [EPR6667]; All lanes : Anti-GCLM antibody [EPR6667] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A673 cell lysate.Lane 3 : PC12 cell lysate.Lane 4 : A431 cell lysate.Lane 5 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 31 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667]; ab126704, at 1/50 dilution, staining GCLM in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Flow Cytometry - Anti-GCLM antibody [EPR6667]; Overlay histogram showing HeLa cells stained with ab126704 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.