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Anti-GCLM Antibody EPR6668
Also for GCLM (NM_002061)
|A synthetic peptide corresponding to residues in human GCLM was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:500; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens glutamate-cysteine ligase, modifier subunit (GCLM)|
|GCLM (Glutamate-cysteine ligase) is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLM deficiency has been implicated in some forms of hemolytic anemia (1). |
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Western blot - Anti-GCLM antibody [EPR6668]; All lanes : Anti-GCLM antibody [EPR6668] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A673 cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated goat anti-rabbit at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 30 kDa .
Other-Anti-GCLM antibody [EPR6668](ab124827); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6668]; ab124827, at 1/100 dilution, staining GCLM in paraffin-embedded Human muscle tissue by Immunohistochemistry.