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Home Antibody All anti-GATA4 antibodies

Anti-GATA4 Antibody EPR4768

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310519
  • Rabbit Monoclonal antibody against Gata4
  • FREE positive control: HEK293T cell transient overexpression lysate (LC419558) , 20ug
100ul 325 3-7 Days
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WB(1)
IF(1)
FC(1)
Assay(1)
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Also for GATA4 (NM_002052)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Gata4 was used as an immunogen.
Clone NameEPR4768 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; FC: 1:1200; ICC/IF: 1:100 - 1:250
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P or IP.

Reference Data

Target NameHomo sapiens GATA binding protein 4 (GATA4)
Alternative NameASD2; TACHD; VSD1
Database LinkNP_002043
FunctionGata4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif. It is thought to regulate proteins involved in embryogenesis and myocardial differentiation and function. Mutations in Gata4 have been associated with cardiac septal defects (1).
Related Pathway
Adipogenesis

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WB Image
Western blot - Anti-GATA4 antibody [EPR4768]; All lanes : Anti-GATA4 antibody [EPR4768] at 1/1000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : Caco-2 cell lysate.Lane 3 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.
Assay Image
Other-Anti-GATA4 antibody [EPR4768](TA310519); Equilibrium disassociation constant (KD)..
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-GATA4 antibody [EPR4768]; Immunofluorescent analysis of HepG2 cells labelling GATA4 with TA310519 at 1/100 dilution.
FC Image
Flow Cytometry - Anti-GATA4 antibody [EPR4768]; Overlay histogram showing HeLa cells stained with TA310519 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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