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Anti-GARS Antibody EPR7157
Also for GARS (NM_002047)
|A synthetic peptide corresponding to residues near the C-terminus of human GARS was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC or IP.
|Homo sapiens glycyl-tRNA synthetase (GARS)|
|CMT2D; DSMAV; GlyRS; HMN5; SMAD1|
|GARS is glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This protein is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis (1). |
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Western blot - Anti-GARS antibody [EPR7157]; All lanes : Anti-GARS antibody [EPR7157] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Hela cell lysate.Lane 3 : U87-MG cell lysate.Lane 4 : 293T cell lysate.Lane 5 : C6 cell lysate.Lane 6 : RAW 264.7 cell lysate.Lane 7 : Neuro-2a cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 83 kDa.Observed band size : 75 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARS antibody [EPR7157]; ab125008 at 1/50 staining GARS in paraffin embedded Human colon tissue by immunohistochemistry.