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Home Antibody All anti-GARS antibodies

Anti-GARS Antibody EPR7157

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310518
  • Rabbit Monoclonal antibody against GARS
  • FREE positive control: HEK293T cell transient overexpression lysate (LC419568) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for GARS (NM_002047)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human GARS was used as an immunogen.
Clone NameEPR7157 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC or IP.

Reference Data

Target NameHomo sapiens glycyl-tRNA synthetase (GARS)
Alternative NameCMT2D; DSMAV; GlyRS; HMN5; SMAD1
Database LinkNP_002038
FunctionGARS is glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This protein is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis (1).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-GARS antibody [EPR7157]; All lanes : Anti-GARS antibody [EPR7157] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Hela cell lysate.Lane 3 : U87-MG cell lysate.Lane 4 : 293T cell lysate.Lane 5 : C6 cell lysate.Lane 6 : RAW 264.7 cell lysate.Lane 7 : Neuro-2a cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 83 kDa.Observed band size : 75 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARS antibody [EPR7157]; TA310518 at 1/50 staining GARS in paraffin embedded Human colon tissue by immunohistochemistry.

 

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