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Anti-EPHA3 PHOSPHO Antibody EPR5684
Also for EPHA3 (NM_005233)
|A phospho specific peptide corresponding to residues surrounding Tyrosine 779 of human EphA3, Tyrosine 779 of EphA4, and Tyrosine 833 of EphA5 was used as an immunogen. This antibody only detects EphA3 phosphorylated at Tyrosine 779, EphA4 phosphorylated at Tyrosine 779, and EphA5 phosphorylated at Tyrosine 833. |
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
|Homo sapiens EPH receptor A3 (EPHA3), transcript variant 1|
|EK4; ETK; ETK1; HEK; HEK4; TYRO4|
|Ephrin receptor tyrosine kinases (RTKs) and their ligands (ephrins) are highly conserved families that mediate cell movement during development (1). There are 14 known receptors, classed into either EphA or EphB, and 8 known ligands, classed into ephrin A or ephrin B. EphA receptors preferably interacts with glycosylphosphatidylinositol-linked ephrins (ephrin A), while EphB receptors preferably interacts with transmembrane ligands (ephrin B) (2). Multiple ligands are specific to each Ephrin receptors and cause receptor activation through transphosphorylation (3, 4). Both receptor and ligand-initiated signals can regulate repulsion, adhesion and de-adhesion mechanisms for the motility of adherent cells (5). Ephrin A3 (EphA3, Mek4, Hek, Hek4) binds to ephrin-A1 to A5 and ephrin-B1. A mutation in EphA3 has been linked to pathogenesis in several tumors, such as breast, lung, and pancreatic cancers (6). Tyrosine 779 of EphA3 is phosphorylated and required to mediate cell de-adhesion upon ephrin-A5 stimulation (7).|
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Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684]; All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] at 1/1000 dilution.Lane 1 : HeLa cell lysate untreated.Lane 2 : HeLa cell lysate treated with Sodium Orthovanadate. See suggested positive control for more treatment information.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 110, 115 kDa.
Other-Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684](ab124881); Equilibrium disassociation constant (KD)..