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Anti-ENAH Antibody EPR5660
Also for ENAH (NM_001008493)
|A synthetic peptide corresponding to residues in human Mena was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; FC: 1:100 - 1:500
|Does not react with Rat. Is unsuitable for ICC or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC or IP.
|Homo sapiens enabled homolog (Drosophila) (ENAH), transcript variant 1|
|ENA; MENA; NDPP1|
|Ena/VASP proteins such as Mena are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity, including axon guidance and lamellipodial and filopodial dynamics in migrating cells. Mena induces the formation of F-actin rich outgrowths in fibroblasts. It acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. In addition, Mena is required for the actin-based mobility of Listeria monocytogenes (1).|
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Western blot - Anti-ENAH antibody [EPR5660]; All lanes : Anti-ENAH antibody [EPR5660] at 1/1000 dilution.Lane 1 : MDA-MB-435 cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : HUVEC cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 67 kDa.Observed band size : 80 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH antibody [EPR5660]; ab124685, at 1/500 dilution, staining ENAH in formalin-fixed, paraffin-embedded Human uterus tissue, by Immunohistochemistry.