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Anti-EIF3E Antibody EPR6878(2)
Also for EIF3E (NM_001568)
|A synthetic peptide corresponding to residues in human eIF-3E was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:250 - 1:500; IP: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens eukaryotic translation initiation factor 3, subunit E (EIF3E)|
|eIF3-p46; EIF3-P48; EIF3S6; INT6|
|eIF-3E is the p48 subunit of the translational initiation factor eIF3. It forms a multi-factor complex with eIF1, eIF5, and the eIF2-GTP-Met-tRNA ternary complex that binds to the 40S ribosomal subunit to form the 43S preinitiation complex (PIC). eIF-3E also prevents the premature joining of the 60S ribosomal subunit until the start codon is recognized. In addition, eIF-3E plays a role in recruiting eIF4G-associated mRNAs to the 43S PIC to form the 48S PIC and initiate mRNA scanning (1). It is required for nonsense-mediated mRNA decay (NMD) and acts in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. eIF-3E also interacts with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (2).|
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Western blot - Anti-eIF3e antibody [6878(2)]; All lanes : Anti-eIF3e antibody [EPR6878(2)] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : 293T cell lysate.Lane 3 : BxPC3 cell lysate.Lane 4 : Saos 2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 52 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3e antibody [6878(2)]; Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling eIF3e with ab134958 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody [6878(2)]; Immunofluorescent analysis of 293T cells labelling eIF3e with ab134958 at 1/250 dilution.