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Home Antibody All anti-EIF3E antibodies

Anti-EIF3E Antibody EPR6878(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310450
  • Rabbit Monoclonal antibody against eIF-3E (EIF3E)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400602) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for EIF3E (NM_001568)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human eIF-3E was used as an immunogen.
Clone NameEPR6878(2) IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:250 - 1:500; IP: 1:10 - 1:100
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 3, subunit E (EIF3E)
Alternative NameeIF3-p46; EIF3-P48; EIF3S6; INT6
Database LinkNP_001559
FunctioneIF-3E is the p48 subunit of the translational initiation factor eIF3. It forms a multi-factor complex with eIF1, eIF5, and the eIF2-GTP-Met-tRNA ternary complex that binds to the 40S ribosomal subunit to form the 43S preinitiation complex (PIC). eIF-3E also prevents the premature joining of the 60S ribosomal subunit until the start codon is recognized. In addition, eIF-3E plays a role in recruiting eIF4G-associated mRNAs to the 43S PIC to form the 48S PIC and initiate mRNA scanning (1). It is required for nonsense-mediated mRNA decay (NMD) and acts in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. eIF-3E also interacts with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (2).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-eIF3e antibody [6878(2)]; All lanes : Anti-eIF3e antibody [EPR6878(2)] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : 293T cell lysate.Lane 3 : BxPC3 cell lysate.Lane 4 : Saos 2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 52 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3e antibody [6878(2)]; Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling eIF3e with TA310450 at 1/50 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody [6878(2)]; Immunofluorescent analysis of 293T cells labelling eIF3e with TA310450 at 1/250 dilution.

 

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