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Anti-EIF3B Antibody EPR5804
Also for EIF3B (NM_001037283)
|A synthetic peptide corresponding to residues in human eIF-3B was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, FC
||WB: 1:10000 - 1:50000; ICC: 1:100 - 1:250; IP: 1:10 - 1:100; FC: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P.
|Homo sapiens eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 2|
|EIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; PRT1|
|eIF-3B is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (1).|
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Western blot - Anti-eIF3B antibody [EPR5804]; All lanes : Anti-eIF3B antibody [EPR5804] at 1/10000 dilution.Lane 1 : A431 cell lysate.Lane 2 : 293T cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : HeLa cell lysate.Lane 5 : Caco 2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 92 kDa.Observed band size : 116 kDa .
Other-Anti-eIF3B antibody [EPR5804](ab133601); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-eIF3B antibody [EPR5804]; Flow cytometric analysis of permeabilized HeLa cells labelling eIF3B with ab133601 at 1/10 (red) with a Rabbit IgG control (green).