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Home Antibody All anti-EIF3B antibodies

Anti-EIF3B Antibody EPR5805

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310447
  • Rabbit Monoclonal antibody against eIF-3B (EIF3B)
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for EIF3B (NM_001037283)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human eIF-3B was used as an immunogen.
Clone NameEPR5805 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF or IP.
Purification pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt,ICC/IF or IP.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 2
Alternative NameEIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; PRT1
Database LinkNP_001032360
Entrez Gene 8662 Human
FunctioneIF-3B is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (1).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-eIF3B antibody [EPR5805]; All lanes : Anti-eIF3B antibody [EPR5805] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : Caco 2 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti Rabbit at 1/2000 dilution.Predicted band size : 92 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3B antibody [EPR5805]; TA310447, at 1/50, staining eIF3B in formalin fixed paraffin embedded Human colon tissue using immunohistochemistry.

 

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