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Anti-EIF3B Antibody EPR5805
Also for EIF3B (NM_001037283)
|A synthetic peptide corresponding to residues in human eIF-3B was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF or IP.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC/IF or IP.
|Homo sapiens eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 2|
|EIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; PRT1|
|eIF-3B is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (1).|
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Western blot - Anti-eIF3B antibody [EPR5805]; All lanes : Anti-eIF3B antibody [EPR5805] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : Caco 2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti Rabbit at 1/2000 dilution.Predicted band size : 92 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3B antibody [EPR5805]; ab124778, at 1/50, staining eIF3B in formalin fixed paraffin embedded Human colon tissue using immunohistochemistry.