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Home Antibody All anti-EIF3A antibodies

Anti-EIF3A Antibody EPR5819(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310446
  • Rabbit Monoclonal antibody against eIF-3A (EIF3A)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401232) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human eIF-3A was used as an immunogen.
Clone NameEPR5819(2) IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 3, subunit A (EIF3A)
Alternative NameEIF3; eIF3-p170; eIF3-theta; EIF3S10; P167; p180; p185; TIF32
Database LinkNP_003741
FunctioneIF-3A belongs to the eIF-3 subunit A family. It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (1).
Related Pathway

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WB Image
Western blot - Anti-eIF3A antibody [EPR5819(2)]; All lanes : Anti-eIF3A antibody [EPR5819(2)] at 1/1000 dilution.Lane 1 : BxPC3 cell lysate.Lane 2 : 293T cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : C6 cell lysate.Lane 5 : RAW264.7 cell lysate.Lane 6 : PC12 cell lysate.Lane 7 : L6 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 166 kDa.
Assay Image
Other-Anti-eIF3A antibody [EPR5819(2)](TA310446); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody [EPR5819(2)]; TA310446, at 1/50 dilution, staining eIF3A in paraffin-embedded Human brain tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody [EPR5819(2)]; TA310446, at 1/250 dilution, staining eIF3A in BxPC3 cells by Immunofluorescence.

 

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