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Anti-EIF3A Antibody EPR5819(2)
Also for EIF3A (NM_003750)
|A synthetic peptide corresponding to residues in human eIF-3A was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for IP.
|Homo sapiens eukaryotic translation initiation factor 3, subunit A (EIF3A)|
|EIF3; eIF3-p170; eIF3-theta; EIF3S10; P167; p180; p185; TIF32|
Entrez Gene 8661 Human
Entrez Gene 13669 Mouse
Entrez Gene 292148 Rat
|eIF-3A belongs to the eIF-3 subunit A family. It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (1).|
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Western blot - Anti-eIF3A antibody [EPR5819(2)]; All lanes : Anti-eIF3A antibody [EPR5819(2)] at 1/1000 dilution.Lane 1 : BxPC3 cell lysate.Lane 2 : 293T cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : C6 cell lysate.Lane 5 : RAW264.7 cell lysate.Lane 6 : PC12 cell lysate.Lane 7 : L6 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 166 kDa.
Other-Anti-eIF3A antibody [EPR5819(2)](TA310446); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody [EPR5819(2)]; TA310446, at 1/50 dilution, staining eIF3A in paraffin-embedded Human brain tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody [EPR5819(2)]; TA310446, at 1/250 dilution, staining eIF3A in BxPC3 cells by Immunofluorescence.