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Anti-DPYSL2 Antibody EPR7792
Also for DPYSL2 (NM_001197293)
|A synthetic peptide corresponding to residues near the C-terminus of human CRMP-2 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, FC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:10 - 1:1000; ICC/IF: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Homo sapiens dihydropyrimidinase-like 2 (DPYSL2), transcript variant 1|
|CRMP-2; CRMP2; DHPRP2; DRP-2; DRP2; N2A3; ULIP-2; ULIP2|
|CRMP-2 is a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. CRMP-2 promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This protein has been implicated in multiple neurological disorders, and hyperphosphorylation of CRMP-2 may play a key role in the development of Alzheimer's disease (1). |
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Western blot - Anti-CRMP2 antibody [EPR7792]; All lanes : Anti-CRMP2 antibody [EPR7792] at 1/10000 dilution.Lane 1 : PC12 cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : U87 MG cell lysate.Lane 4 : NIH 3T3 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 62 kDa.Observed band size : 64 kDa .
Other-Anti-CRMP2 antibody [EPR7792](TA310419); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR7792]; TA310419, at a dilution of 1/100, staining CRMP2 in paraffin embedded Human astrocytoma tissue by Immunohistochemistry.
Flow Cytometry - Anti-CRMP2 antibody [EPR7792]; Overlay histogram showing SH-SY5Y cells stained with TA310419 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.