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Home Antibody All anti-DHFR antibodies

Anti-DHFR Antibody EPR5285

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310408
  • Rabbit Monoclonal antibody against DHFR
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400271) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
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Also for DHFR (NM_000791)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human DHFR was used as an immunogen.
Clone NameEPR5285 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; ICC: 1:25 - 1:50
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens dihydrofolate reductase (DHFR), transcript variant 1
Alternative NameDHFRP1; DYR
Database LinkNP_000782
FunctionDihydrofolate reductase (DHFR) converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR deficiency has been linked to megaloblastic anemia (1).
Related Pathway
Cell cycle

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]; All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] at 1/10000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : Jurkat cell lysates.Lane 3 : Y-79 cell lysates.Lane 4 : 293T cell lysates.Lane 5 : Mouse spleen lysates.Lane 6 : Rat kidney lysates.Lysates/proteins at 10 µg per lane.Predicted band size : 21 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]; TA310408 at 1/100 dilution, staining Dihydrofolate reductase (DHFR) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

 

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