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Anti-DCTN3 Antibody EPR5097
Also for DCTN3 (NM_007234)
|A synthetic peptide corresponding to residues in human DCTN3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC or IP.
|Homo sapiens dynactin 3 (p22) (DCTN3), transcript variant 1|
|DCTN3 is the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. DCTN3 is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin (1).|
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Western blot - Anti-DCTN3 antibody [EPR5097]; All lanes : Anti-DCTN3 antibody [EPR5097] at 1/1000 dilution.Lane 1 : 293T cell lysates.Lane 2 : Mouse muscle lysates.Lane 3 : HeLa cell lysates.Lane 4 : BxPC 3 cell lysates.Lane 5 : SH-SY5Y cell lysates.Lane 6 : Rat muscle lysates.Lysates/proteins at 10 µg per lane.Secondary.Lanes 1, 2, 3, 5 & 6 : Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 21 kDa.
Other-Anti-DCTN3 antibody [EPR5097](ab124674); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097]; ab124674, at 1/100 dilution, staining DCTN3 in paraffin embedded Human breast tissue by Immunohistochemistry.