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Anti-DCTN2 Antibody EPR5095
Also for DCTN2 (NM_006400)
|A synthetic peptide corresponding to residues in human DCTN2 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250; IP: 1:10 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens dynactin 2 (p50) (DCTN2), transcript variant 1|
|DCTN50; DYNAMITIN; RBP50|
|DCTN2 is a subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. DCTN2 contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place (1).|
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Western blot - Anti-Dynamitin antibody [EPR5095]; All lanes : Anti-Dynamitin antibody [EPR5095] at 1/1000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : A375 cell lysate.Lane 3 : Saos2 cell lysate.Lane 4 : SH SY5Y cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 44 kDa.Observed band size : 44 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-Dynamitin antibody [EPR5095]; Immunofluorescence analysis of Dynamitin in SH-SY5Y cells, using ab133492 at a dilution of 1/100.