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Anti-DAP12 Antibody EPR5173


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal antibody against DAP12
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418750) , 20ug Explanation
100ul $325 3-7 Days

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

Also for DAP12 (NM_003332)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human DAP12 was used as an immunogen.
Clone NameEPR5173 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsIHC-Fr: 1:500; WB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IHC-P or IP.

Reference Data

Target NameHomo sapiens TYRO protein tyrosine kinase binding protein (TYROBP), transcript variant 1
Alternative NameDAP12; KARAP; PLOSL
Database LinkNP_003323
Entrez Gene 7305 Human
FunctionDAP12 is a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. It may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. DAP12 may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this protein have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL (1).
Related PathwayTransmembraneDruggable Genome Natural killer cell mediated cytotoxicity

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Western blot - Anti-DAP12 antibody [EPR5173]; All lanes : Anti-DAP12 antibody [EPR5173] at 1/1000 dilution.Lane 1 : Peripheral blood leukocytes lysates.Lane 2 : Human placenta lysates.Lane 3 : Peripheral blood mononuclear lysates.Lysates/proteins at 10 ug per lane.Predicted band size : 12 kDa.
Other-Anti-DAP12 antibody [EPR5173](TA310389); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Frozen sections) - Anti-DAP12 antibody [EPR5173]; IHC-Fr image of human blood vessels stained with Ab124834 (1/500). The fixed brain sectionwere permeabilise in 0.1% PBS-Triton X for 1h. The sections were incubated with 10% donkey serum for 1 h at 24°C to block non-specific protein-protein interactions. The sections were then incubated with Ab124834 used at 1/500 dilution, overnight at 4°C. The secondary antibody was Alexa Fluor? 488 donkey anti-rabbit used at a 1/1000 dilution for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.


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