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Anti-DAP12 Antibody EPR5173
Also for DAP12 (NM_003332)
|A synthetic peptide corresponding to residues in human DAP12 was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC
||IHC-Fr: 1:500; WB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for IHC-P or IP.
|Homo sapiens TYRO protein tyrosine kinase binding protein (TYROBP), transcript variant 1|
|DAP12; KARAP; PLOSL|
Entrez Gene 7305 Human
|DAP12 is a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. It may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. DAP12 may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this protein have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL (1). |
|TransmembraneDruggable Genome Natural killer cell mediated cytotoxicity|
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Western blot - Anti-DAP12 antibody [EPR5173]; All lanes : Anti-DAP12 antibody [EPR5173] at 1/1000 dilution.Lane 1 : Peripheral blood leukocytes lysates.Lane 2 : Human placenta lysates.Lane 3 : Peripheral blood mononuclear lysates.Lysates/proteins at 10 ug per lane.Predicted band size : 12 kDa.
Other-Anti-DAP12 antibody [EPR5173](TA310389); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Frozen sections) - Anti-DAP12 antibody [EPR5173]; IHC-Fr image of human blood vessels stained with Ab124834 (1/500). The fixed brain sectionwere permeabilise in 0.1% PBS-Triton X for 1h. The sections were incubated with 10% donkey serum for 1 h at 24Â°C to block non-specific protein-protein interactions. The sections were then incubated with Ab124834 used at 1/500 dilution, overnight at 4Â°C. The secondary antibody was Alexa Fluor? 488 donkey anti-rabbit used at a 1/1000 dilution for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.