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Anti-CYBB Antibody EPR6991
Also for CYBB (NM_000397)
|A synthetic peptide corresponding to residues in human gp91-phox was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, FC
||FC: 1:100 - 1:1000; WB: 1:1000 - 1:10000
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens cytochrome b-245, beta polypeptide (CYBB)|
|AMCBX2; CGD; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX|
Entrez Gene 1536 Human
Entrez Gene 13058 Mouse
Entrez Gene 66021 Rat
|gp91-phox, or Cytochrome b (-245), is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole (1). |
|Ion Channels: OtherTransmembraneDruggable Genome Leukocyte transendothelial migration|
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Western blot - Anti-NOX2/gp91phox antibody [EPR6991]; All lanes : Anti-NOX2/gp91phox antibody [EPR6991] at 1/1000 dilution.Lane 1 : MCF-7 cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : Caco-2 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 65 kDa.Observed band size : 60 kDa .
Other-Anti-NOX2/gp91phox antibody [EPR6991](TA310385); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-NOX2/gp91phox antibody [EPR6991]; Overlay histogram showing HepG2 cells stained with TA310385 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.