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Anti-Chk2 Antibody EPR5528
Also for Chk2 (NM_001005735)
|A synthetic peptide corresponding to residues at the N-terminus of human Chk2 was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: Use at an assay dependent dilution
|pH: 7.40Preservative: 0.01% Sodium azide; Constituents: 50% Glycerol, 0.05% BSA|
|Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
|Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 3|
|CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53|
Entrez Gene 11200 Human
|Chk2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, Chk2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in Chk2 have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this protein are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors (1).|
|Protein KinaseStem cell - PluripotencyDruggable Genome Cell cyclep53 signaling pathway|
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Western blot - Anti-Chk2 antibody [EPR5528]; All lanes : Anti-Chk2 antibody [EPR5528] at 1/10000 dilution.Lane 1 : SH-SY5Y lysate.Lane 2 : HeLa lysate.Lane 3 : HT29 lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat-anti-rabbit HRP at 1/2000 dilution.Predicted band size : 61 kDa.Observed band size : 61 kDa.
Other-Anti-Chk2 antibody [EPR5528](TA310327); Equilibrium disassociation constant (KD)..