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Anti-Chk2 PHOSPHO Antibody EPR916(2)Y
Also for Chk2 (NM_145862)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 33/35 of human Chk2 was used as immunogen. The antibody only detects Chk2 phosphorylated on Serine 33/35.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:2,000-20,000; IHC 1:100; ICC: 1:100 - 250;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 2|
|CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53|
|Chk2 is a checkpoint kinase that plays a role in regulating cell cycle progression. In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (1,2). Consisting of ionizing radiation (IR), UV irradiation or hydroxyurea treatment, DNA damage induces ATM/ATR to phosphorylate Thr68 and other sites in the amino terminal domain (3). Phosphorylation at Thr68 is a prerequisite for subsequent activation steps.|
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Western blot - Chk2 (phospho S33 + S35) antibody [EPR916(2)Y]; All lanes : Anti-Chk2 (phospho S33 + S35) antibody [EPR916(2)Y] at 1/500 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with irradiation.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated Goat anti-rabbit at 1/2000 dilution.Predicted band size : 61 kDa.Observed band size : 61 kDa.