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Home Antibody All anti-CD4 antibodies

Anti-CD4 Antibody EPR6855

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA310278
  • Rabbit Monoclonal antibody against CD4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400209) , 20ug Explanation
100ul $325 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(6)
FC(1)

OriGene Data

ImmunogenA recombinant protein fragment corresponding to residues in human CD4 was used as an immunogen.
Clone NameEPR6855 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:100
BufferpH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens CD4 molecule (CD4), transcript variant 1
Alternative NameCD4mut
Database LinkNP_000607
Entrez Gene 920 Human
FunctionT-cell surface glycoprotein CD4 (CD4) is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It regulates T-cell activation, partially by recruiting the tyrosine kinase Lck, and induces the aggregation of lipid rafts. CD4 binds to HIV-1 gp120 and to P4HB/PDI, and upon HIV-1 binding to the cell membrane, is part of the P4HB/PDI-CD4-CXCR4-gp120 complex (1).
Related PathwayES Cell Differentiation/IPSTransmembraneAdult stem cellsInduced pluripotent stem cellsDruggable Genome Cell adhesion molecules (CAMs)Antigen processing and presentationHematopoietic cell lineageT cell receptor signaling pathwayPrimary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-CD4 antibody [EPR6855]; All lanes : Anti-CD4 antibody [EPR6855] at 1/1000 dilution.Lane 1 : THP1 cell lysate.Lane 2 : Human fetal thymus lysate.Lane 3 : Human tonsil lysate.Lane 4 : Human lymph node lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 51 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855]; Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD4 with TA310278 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855]; Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling CD4 with TA310278 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Normal liver tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Normal spleen tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Normal colon tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Thymoma tissue.
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Flow Cytometry - Anti-CD4 antibody [EPR6855]; Human peripheral blood lymphocytes stained with TA310278 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody for 30 min at 4°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
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