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Home Antibody All anti-CD4 antibodies

Anti-CD4 Antibody EPR6855

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310278
  • Rabbit Monoclonal antibody against CD4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400209) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(6)
FC(1)
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Also for CD4 (NM_000616)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA recombinant protein fragment corresponding to residues in human CD4 was used as an immunogen.
Clone NameEPR6855 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens CD4 molecule (CD4), transcript variant 1
Alternative NameCD4mut
Database LinkNP_000607
Entrez Gene 920 Human
FunctionT-cell surface glycoprotein CD4 (CD4) is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It regulates T-cell activation, partially by recruiting the tyrosine kinase Lck, and induces the aggregation of lipid rafts. CD4 binds to HIV-1 gp120 and to P4HB/PDI, and upon HIV-1 binding to the cell membrane, is part of the P4HB/PDI-CD4-CXCR4-gp120 complex (1).
Related PathwayES Cell Differentiation/IPSTransmembraneAdult stem cellsInduced pluripotent stem cellsDruggable Genome Cell adhesion molecules (CAMs)Antigen processing and presentationHematopoietic cell lineageT cell receptor signaling pathwayPrimary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-CD4 antibody [EPR6855]; All lanes : Anti-CD4 antibody [EPR6855] at 1/1000 dilution.Lane 1 : THP1 cell lysate.Lane 2 : Human fetal thymus lysate.Lane 3 : Human tonsil lysate.Lane 4 : Human lymph node lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 51 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855]; Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD4 with TA310278 at 1/100 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855]; Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling CD4 with TA310278 at 1/100 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Normal liver tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Normal spleen tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Normal colon tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Thymoma tissue.
FC Image
Flow Cytometry - Anti-CD4 antibody [EPR6855]; Human peripheral blood lymphocytes stained with TA310278 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody for 30 min at 4°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

 

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