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Anti-CD151 Antibody EP6875
Also for CD151 (NM_001039490)
|A synthetic peptide corresponding to residues at the N-terminus of human CD151 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; FC: 1:100 - 1:1000
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for ICC or IP.
|Homo sapiens CD151 molecule (Raph blood group) (CD151), transcript variant 5|
|GP27; MER2; PETA-3; RAPH; SFA1; TSPAN24|
Entrez Gene 977 Human
Entrez Gene 12476 Mouse
Entrez Gene 64315 Rat
|CD151 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD151 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells (1). |
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Western blot - Anti-CD151 antibody [EP6875]; All lanes : Anti-CD151 antibody [EP6875] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : K562 cell lysate.Lane 3 : TF-1 cell lysate.Lane 4 : Saos 2 cell lysate.Lysates/proteins at 10 ug/ml per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : 32 kDa .
Other-Anti-CD151 antibody [EP6875](TA310256); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EP6875]; Immunohistochemical analysis of paraffin embedded Human kidney tissue labelled with TA310256 antibody at 1/250 dilution.
Flow Cytometry - Anti-CD151 antibody [EP6875]; Overlay histogram showing K562 cells stained with TA310256 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.