Anti-CBL Antibody, EPR2227

Also for CBL (NM_005188)

OriGene Data

Immunogen	A phospho specific peptide corresponding to residues surrounding tyrosine 674 of human Cbl were used as an immunogen. This antibody detects Cbl phosphorylated on tyrosine 674.
Clone Name	EPR2227	Isotype
Species Reactivity	Human	Concentration
Guaranteed Application *	WB	Suggested Dilutions	WB: 1:1000 - 2000;
Buffer	Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens Cbl proto-oncogene, E3 ubiquitin protein ligase (CBL)
Alternative Name	C-CBL; CBL2; FRA11B; NSLL; RNF55
Database Link	NP_005179
Function	c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4). It has been demonstrated that certain tyrosines are the major phosphorylation sites of c-Cbl in T cells in response to pervanadate treatment, as well as in response to TcR/CD3 ligation. Cbl also enhances migration of v-Abl-transformed NIH 3T3 fibroblasts. This effect of Cbl depends on its tyrosine phosphorylation, which is required for binding of PI-3' kinase to c-Cbl (5)
Related Pathway	EGFR1 Signaling Pathway Jak-STAT signaling pathway

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WB Image

A. Western blot analysis on Jurkat cell lysates using anti-Phospho-Cbl (pY674) RabMAb, 1:1000 dilution. Cells were either (A) untreated (B) treated with pervanadate.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
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FITC
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DyLight® 633

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OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-CBL Phospho Antibody EPR2227

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data