Home All anti-CBL antibodies
Anti-CBL Phospho Antibody EPR2227
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
|A phospho specific peptide corresponding to residues surrounding tyrosine 674 of human Cbl were used as an immunogen. This antibody detects Cbl phosphorylated on tyrosine 674.|
||WB: 1:1000 - 2000;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens Cbl proto-oncogene, E3 ubiquitin protein ligase (CBL)|
|C-CBL; CBL2; FRA11B; NSLL; RNF55|
|c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4). It has been demonstrated that certain tyrosines are the major phosphorylation sites of c-Cbl in T cells in response to pervanadate treatment, as well as in response to TcR/CD3 ligation. Cbl also enhances migration of v-Abl-transformed NIH 3T3 fibroblasts. This effect of Cbl depends on its tyrosine phosphorylation, which is required for binding of PI-3' kinase to c-Cbl (5)|
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-Cbl (pY674) RabMAb, 1:1000 dilution. Cells were either (A) untreated (B) treated with pervanadate.