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Anti-CBL PHOSPHO Antibody EP973Y
|A synthetic phospho-peptide corresponding to residues surrounding Tyr731 of human Cbl was used as immunogen. The antibody detects Cbl phosphorylated on Tyrosine 731.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1,000 - 10,000; IHC 1:250 - 500; ICC: 1:250 - 500; IP: 1:50; FC: 1:50;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens Cbl proto-oncogene, E3 ubiquitin protein ligase (CBL)|
|C-CBL; CBL2; FRA11B; NSLL; RNF55|
|c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).|
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
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All lanes : Anti-CBL (phospho Y731) antibody [EP973Y] (TA310243) at 1/2000 dilution. Lane 1 : Raji cell lysate untreated; Lane 2 : Raji cell lysate treated with pervanadate. 10ug lysate/protein per lane. Secondary: goat anti-rabbit HRP at 1/2000 dilution.