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Anti-BUB3 Antibody EPR5320(2)
Also for BUB3 (NM_001007793)
|A synthetic peptide corresponding to residues in human Bub3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens BUB3 mitotic checkpoint protein (BUB3), transcript variant 2|
|Bub3 has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. It promotes the formation of stable end-on bipolar attachments. and is necessary for kinetochore localization of BUB1. Bub3 regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20 (1).|
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Western blot - Anti-Bub3 antibody [EPR5320(2)]; All lanes : Anti-Bub3 antibody [EPR5320(2)] at 1/1000 dilution.Lane 1 : Hela cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 37 kDa.Observed band size : 40 kDa .
Other-Anti-Bub3 antibody [EPR5320(2)](ab131157); Equilibrium disassociation constant (KD)..