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Home Antibody All anti-BRD4 antibodies

Anti-BRD4 Antibody EPR5150(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310215
  • Rabbit Monoclonal antibody against BRD4
  • FREE positive control: HEK293T cell transient overexpression lysate (LC415375) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human BRD4 was used as an immunogen.
Clone NameEPR5150(2) IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:250 - 1:500
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens bromodomain containing 4 (BRD4), transcript variant short
Alternative NameCAP; HUNK1; HUNKI; MCAP
Database LinkNP_055114
FunctionBRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif that may be involved in chromatin targeting. BRD4 has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people (1).
Related Pathway

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WB Image
Western blot - Anti-Brd4 antibody [EPR5150(2)]; All lanes : Anti-Brd4 antibody [EPR5150(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Caco2 cell lysate.Lane 3 : T.T. cell lysate.Lane 4 : RAW264.7 cell lysate.Lane 5 : NIH 3T3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 152 kDa.
Assay Image
Other-Anti-Brd4 antibody [EPR5150(2)](TA310215); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [EPR5150(2)]; TA310215 at 1/100 dilution staining Brd4 in Human colon carcinoma tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)]; TA310215 (1/500) staining Brd4 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counter stained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

 

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