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Home Antibody All anti-BRD4 antibodies

Anti-BRD4 Antibody EPR5150(2)

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA310215 Rabbit Monoclonal antibody against BRD4 100ul $325 In Stock
LC415375 BRD4 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(1)
IF(1)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human BRD4 was used as an immunogen.
Clone NameEPR5150(2) IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:250 - 1:500
Predicted MW Explanation 152 kDa
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens bromodomain containing 4 (BRD4), transcript variant short
Alternative NameCAP; HUNK1; HUNKI; MCAP
Database LinkNP_055114
Entrez Gene 23476 Human
Entrez Gene 57261 Mouse
Entrez Gene 362844 Rat
FunctionBRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif that may be involved in chromatin targeting. BRD4 has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people (1).
Related PathwayProtein Kinase

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-Brd4 antibody [EPR5150(2)]; All lanes : Anti-Brd4 antibody [EPR5150(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Caco2 cell lysate.Lane 3 : T.T. cell lysate.Lane 4 : RAW264.7 cell lysate.Lane 5 : NIH 3T3 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 152 kDa.
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Other-Anti-Brd4 antibody [EPR5150(2)](TA310215); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [EPR5150(2)]; TA310215 at 1/100 dilution staining Brd4 in Human colon carcinoma tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)]; TA310215 (1/500) staining Brd4 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counter stained with DAPI in order to highlight the nucleus (red).
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