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Anti-ATG3 Antibody EPR4802
Also for ATG3 (NM_022488)
|A synthetic peptide corresponding to residues on the C-terminus in human Atg3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC/IF or IHC-P.
|Homo sapiens autophagy related 3 (ATG3), transcript variant 1|
|APG3; APG3-LIKE; APG3L; PC3-96|
|Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole (1). Atg3 is an E2-like enzyme involved in autophagy and mitochondrial homeostasis. It catalyzes the conjugation of Atg8-like proteins to phosphatidylethanolamine (PE), which is essential to autophagy. Atg3 also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of Atg12 to itself; Atg12 conjugation to Atg3 plays a role in mitochondrial homeostasis but not in autophagy. Atg7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with Atg3 (2).|
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Western blot - Apg3 antibody [EPR4802]; All lanes : Anti-Apg3 antibody [EPR4802] at 1/1000 dilution.Lane 1 : K562 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 36 kDa.Observed band size : 40 kDa .
Flow Cytometry - Apg3 antibody [EPR4802]; Flow cytometric analysis of permeabilized Jurkat cells using ab108282 at 1/10 (red) or a rabbit IgG (negative) (green).