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Home Antibody All anti-ATG3 antibodies

Anti-ATG3 Antibody EPR4801

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310202
  • Rabbit Monoclonal antibody against ATG3
  • FREE positive control: HEK293T cell transient overexpression lysate (LC411559) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for ATG3 (NM_022488)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus in human Atg3 was used as an immunogen.
Clone NameEPR4801 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens autophagy related 3 (ATG3), transcript variant 1
Alternative NameAPG3; APG3-LIKE; APG3L; PC3-96
Database LinkNP_071933
FunctionAutophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole (1). Atg3 is an E2-like enzyme involved in autophagy and mitochondrial homeostasis. It catalyzes the conjugation of Atg8-like proteins to phosphatidylethanolamine (PE), which is essential to autophagy. Atg3 also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of Atg12 to itself; Atg12 conjugation to Atg3 plays a role in mitochondrial homeostasis but not in autophagy. Atg7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with Atg3 (2).
Related Pathway

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WB Image
Western blot - Apg3 antibody [EPR4801]; All lanes : Anti-Apg3 antibody [EPR4801] at 1/10000 dilution.Lane 1 : K562 cell lysate.Lane 2 : HL-60 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 2000 .Predicted band size : 36 kDa.Observed band size : 40 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apg3 antibody [EPR4801]; TA310202, at a 1/100 dilution, staining Human Apg3 in muscle, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Apg3 antibody [EPR4801]; Immunofluorescent staining of HeLa cells using TA310202 at 1/100.

 

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