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Anti-ATG3 Antibody EPR4801
Also for ATG3 (NM_022488)
|A synthetic peptide corresponding to residues near the N-terminus in human Atg3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens autophagy related 3 (ATG3), transcript variant 1|
|APG3; APG3-LIKE; APG3L; PC3-96|
|Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole (1). Atg3 is an E2-like enzyme involved in autophagy and mitochondrial homeostasis. It catalyzes the conjugation of Atg8-like proteins to phosphatidylethanolamine (PE), which is essential to autophagy. Atg3 also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of Atg12 to itself; Atg12 conjugation to Atg3 plays a role in mitochondrial homeostasis but not in autophagy. Atg7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with Atg3 (2).|
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Western blot - Apg3 antibody [EPR4801]; All lanes : Anti-Apg3 antibody [EPR4801] at 1/10000 dilution.Lane 1 : K562 cell lysate.Lane 2 : HL-60 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 2000 .Predicted band size : 36 kDa.Observed band size : 40 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apg3 antibody [EPR4801]; ab108251, at a 1/100 dilution, staining Human Apg3 in muscle, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Apg3 antibody [EPR4801]; Immunofluorescent staining of HeLa cells using ab108251 at 1/100.