Anti-ATG3 Antibody EPR4801

SKU	Description	Amount	Price	Availability*
TA310202	Rabbit Monoclonal antibody against ATG3 FREE positive control: HEK293T cell transient overexpression lysate (LC411559) , 20ug	100ul	$325	3-7 Days
TA802519S	Purified GAPDH mouse monoclonal antibody (Loading control), clone 2D9	30ul	$100 $50*	In Stock

* Buy one primary antibody, get 50% off a GAPDH loading control antibody.

WB(1)

IHC(1)

IF(1)

Also for ATG3 (NM_022488)

OriGene Data

Immunogen	A synthetic peptide corresponding to residues near the N-terminus in human Atg3 was used as an immunogen.
Clone Name	EPR4801	Isotype
Species Reactivity	Human, Mouse, Rat	Concentration
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB: 1:1,000-10,000; FC: 1:1800;
Buffer	Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens autophagy related 3 (ATG3)
Alternative Name	APG3; APG3-LIKE; APG3L; PC3-96
Database Link	NP_071933
Function	Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole (1). Atg3 is an E2-like enzyme involved in autophagy and mitochondrial homeostasis. It catalyzes the conjugation of Atg8-like proteins to phosphatidylethanolamine (PE), which is essential to autophagy. Atg3 also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of Atg12 to itself; Atg12 conjugation to Atg3 plays a role in mitochondrial homeostasis but not in autophagy. Atg7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with Atg3 (2).
Related Pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

Western blot analysis on (A) K562, (B) HL-60, (C) HeLa, and (D) Jurkat cell lysates using anti-Atg3 RabMAb.

IHC Image

Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-Atg3 RabMAb.

IF Image

Immunofluorescent staining of HeLa cells using anti-Atg3 RabMAb.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

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Anti-ATG3 Antibody EPR4801

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data