Homo sapiens AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 1
Synonyms:
Lobe; PRAS40
Immunogen
A phospho specific peptide corresponding to residues surrounding Threonine 246 of human PRAS40 was used as an immunogen. This antibody only detects PRAS40 phosphorylated at Threonine 246.
Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
PRAS40 is a novel substrate of Akt, the phosphorylation of which leads to the binding of this protein to 14-3-3 (1). It is a subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1 (2).
Related Pathway
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Western blot analysis on HeLa cell lysates using anti-Phospho-PRAS40 (pT246) RabMAb. Cells were either (A) untreated or (B) treated with Lambda Phosphatase.
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