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Anti-AKT1S1 PHOSPHO Antibody EPR6262-107
Also for AKT1S1 (NM_032375)
|A phospho specific peptide corresponding to residues surrounding Threonine 246 of human PRAS40 was used as an immunogen. This antibody only detects PRAS40 phosphorylated at Threonine 246.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100
|Does not react with Rat. Is unsuitable for ICC/IF or IHC-P.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for ICC/IF or IHC-P.
|Homo sapiens AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 1|
|PRAS40 is a novel substrate of Akt, the phosphorylation of which leads to the binding of this protein to 14-3-3 (1). It is a subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1 (2).|
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Western blot - Anti-PRAS40 (phospho T246) antibody [EPR6262]; All lanes : Anti-PRAS40 (phospho T246) antibody [EPR6262-107] at 1/1000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with Lambda Phosphatase.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-rabbit at 1/2000 dilution.Predicted band size : 27 kDa.