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Anti-AKT1S1 Antibody EPR6262
Also for AKT1S1 (NM_032375)
|A synthetic peptide corresponding to residues near the C-terminus of human PRAS40 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC or IHC-P.
|Homo sapiens AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 1|
|PRAS40 is a novel substrate of Akt, the phosphorylation of which leads to the binding of this protein to 14-3-3 (1). It is a subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1 (2).|
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Western blot - Anti-PRAS40 antibody [EPR6262]; All lanes : Anti-PRAS40 antibody [EPR6262] at 1/1000 dilution.Lane 1 : NIH/3T3 cell lysate.Lane 2 : L6 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 27 kDa.