Anti-AKT1S1 Antibody, EPR6262

Also for AKT1S1 (NM_032375)

Gene Name	Homo sapiens AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 1
Synonyms:	Lobe; PRAS40
Immunogen	A synthetic peptide corresponding to residues near the C-terminus of human PRAS40 was used as an immunogen.
Buffer	Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Clone Name	EPR6262	Isotype
Species Reactivity	Human, Mouse, Rat	Concentration
Purification	(Protein A or G Sepharose)
Guaranteed Application *	WB	Suggested Dilutions	WB: 1:1,000 - 10,000; IHC 1:50 - 100; IP: 1:10 - 100;
Background	PRAS40 is a novel substrate of Akt, the phosphorylation of which leads to the binding of this protein to 14-3-3 (1). It is a subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1 (2).
Related Pathway

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WB Image

Western blot analysis on (A) NIH/3T3, (B) L6, (C) HeLa, and (D) 293T cell lysates using anti-PRAS40 RabMAb.

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Anti-AKT1S1 Antibody EPR6262

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