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Home Antibody All anti-ACTA2 antibodies

Anti-ACTA2 Antibody EPR5368

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310169
  • Rabbit Monoclonal antibody against Alpha-Actin (Smooth Muscle) (ACTA2)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC427983) , 20ug
100ul 325 In Stock
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WB(1)
IHC(8)
Assay(1)
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Also for ACTA2 (NM_001141945)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus in human Alpha-Actin (Smooth Muscle) was used as an immunogen.
Clone NameEPR5368 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsIHC-P: 1:1000 - 1:2500; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for ,IP or WB.

Reference Data

Target NameHomo sapiens actin, alpha 2, smooth muscle, aorta (ACTA2), transcript variant 1
Alternative NameAAT6; ACTSA; MYMY5
Database LinkNP_001135417
FunctionActins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4). Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Furthermore, expression of smooth muscle alpha actin is regulated by hormones and cell proliferation, and is altered by pathological conditions including oncogenic transformation and atherosclerosis (5).
Related Pathway

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WB Image
Western blot - Anti-alpha smooth muscle Actin antibody (TA310169) at 1:1000. Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue; Lane 2 : Heart (Mouse) Tissue Lysate; Lane 3 : Heart (Rat) Tissue Lysate; Lane 4 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue; Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate; Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate; Lane 7 : SV40LT-SMC (Rat aorta smooth muscle) Whole Cell Lysate. Lysates/proteins at 10 µg per lane. (Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1:1000 dilution; developed using the ECL technique; exposure time: 30 seconds)
Assay Image
Other-Anti-alpha smooth muscle Actin antibody [EPR5368](TA310169); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368]; TA310169, at 1/1000 dilution, staining alpha smooth muscle Actin in paraffin embedded Human heart tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368]; TA310169, at 1/1000 dilution, staining alpha smooth muscle Actin in paraffin embedded Human ovary tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-alpha smooth muscle Actin antibody [EPR5368](TA310169); TA310169 showing positive staining in Normal liver vessels tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-alpha smooth muscle Actin antibody [EPR5368](TA310169); TA310169 showing positive staining in Normal colon smooth muscles tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-alpha smooth muscle Actin antibody [EPR5368](TA310169); TA310169 showing positive staining in Prostatic carcinoma smooth muscles tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-alpha smooth muscle Actin antibody [EPR5368](TA310169); TA310169 showing positive staining in Skeletal muscle vessels tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-alpha smooth muscle Actin antibody [EPR5368](TA310169); TA310169 showing positive staining in Stomach carcinoma smooth muscles tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368]; TA310169 showing positive staining in Normal tonsil vessels tissue.

 

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